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Study Information
Study Attribute Value
CEBS Accession Number: 002-03083-0002-0000-8
Chemical Name: m-Phenylenediamine
CASRN: 108-45-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 434853_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
Induced Rat Liver S9  1_S9  Negative 
No Activation  2  Positive 

Trial #:1   Activation: No Activation    Date: 12/18/1985    Harvest Time: 20 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 1 0.005 0.5 0 0.000 0.0 1 0.005 0.5 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 200 4 0.020 1.5 1 0.005 0.5 3 0.015 1.5 0 0.000 0.0
Test Chemical: m-Phenylenediamine 
246.5       
 100 75 0.750 31.0 9 0.090 5.0 46 0.460 28.0 20 0.200 2.0 
502.5       
 100 85 0.850 32.0 12 0.120 11.0 43 0.430 24.0 30 0.300 3.0 
747         
 25 50 2.000 64.0 6 0.240 12.0 24 0.960 52.0 20 0.800 8.0 
1000         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.05      
 200 63 0.315 19.5 22 0.110 9.5 41 0.205 14.5 0 0.000 0.0
Mitomycin-C 
 0.08      
 25 46 1.840 64.0 6 0.240 20.0 30 1.200 56.0 10 0.400 4.0
Trend: 8.627 4.406 7.128  
Probability: 0.000 0.000 0.000  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 12/18/1985    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 6 0.030 2.5 0 0.000 0.0 6 0.030 2.5 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 200 5 0.025 2.5 2 0.010 1.0 3 0.015 1.5 0 0.000 0.0
Test Chemical: m-Phenylenediamine 
2513         
 200 13 0.065 4.5 5 0.025 2.5 8 0.040 2.5 0 0.000 0.0 
3750         
 200 7 0.035 2.5 2 0.010 1.0 5 0.025 2.0 0 0.000 0.0 
5000         
 200 15 0.075 6.0 7 0.035 3.5 8 0.040 3.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
7.5       
 200 17 0.085 7.0 3 0.015 1.5 14 0.070 5.5 0 0.000 0.0
Cyclophosphamide 
37.5       
 25 9 0.360 28.0 4 0.160 12.0 5 0.200 16.0 0 0.000 0.0
Trend: 1.295 1.258 0.821  
Probability: 0.098 0.104 0.206  


Trial #:2   Activation: No Activation    Date: 01/23/1986    Harvest Time: 20 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 3 0.030 3.0 2 0.020 2.0 1 0.010 1.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 3 0.030 3.0 0 0.000 0.0 3 0.030 3.0 0 0.000 0.0
Test Chemical: m-Phenylenediamine 
251.3       
 100 56 0.560 23.0 6 0.060 6.0 20 0.200 16.0 30 0.300 3.0 
502.5       
 25 39 1.560 44.0 6 0.240 16.0 23 0.920 40.0 10 0.400 4.0 
750         
 50 58 1.160 48.0 10 0.200 14.0 38 0.760 40.0 10 0.200 2.0 
1000         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.05      
 100 41 0.410 30.0 16 0.160 14.0 25 0.250 19.0 0 0.000 0.0
Mitomycin-C 
 0.08      
 25 30 1.200 52.0 10 0.400 28.0 20 0.800 40.0 0 0.000 0.0
Trend: 6.761 3.850 6.260  
Probability: 0.000 0.000 0.000