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Study Information
Study Attribute Value
CEBS Accession Number: 002-02981-0006-0000-8
Chemical Name: Trisodium ethylenediaminetetraacetate trihydrate (EDTA)
CASRN: 150-38-9
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 127205
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water
 0         
64# 92 148.5 78 69
    65 108 133 68
    63 112 132 70
    64 87 113 59
Test Chemical Trisodium ethylenediaminetetraacetate trihydrate (EDTA)
60         
65 107 177 91 83
    74 109 165 74
 
70         
67 113 162 81 68
    58 110 97 56
 
80         
67 100 131 65 59
    63# 116 99 52
 
90         
58 86 119 69 72
    63 93 140 75
 
100         
71 111 142 67 73
    66 101 159 80
Positive Control Methyl Methane Sulfonate
15         
49 42 293 198 181*
    53 45 261 164

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium
 0         
84 102 63 25 18
    103 104 57 18
    93 91 50 18
    78 103 24 10
Test Chemical Trisodium ethylenediaminetetraacetate trihydrate (EDTA)
1000         
76 51 45 20 23
    80 42 61 25
 
2000         
87 53 52 20 21
    88 52 61 23
 
3000         
79 38 47 20 21
    77 50 50 22
 
4000         
65 30 49 25 27
    93 32 80 29
 
5000         
76 29 54 24 22
    79 23 49 21
Positive Control Methyl Methane Sulfonate
15         
38 18 107 93 93*
    52 25 146 93

Nonactivation Trial: 3 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium
 0         
69 94 88 43 38
    62 109 48 26
    58 87 79 46
    76 110 84 37
Test Chemical Trisodium ethylenediaminetetraacetate trihydrate (EDTA)
1000         
61 59 95 52 57
    55 60 101 61
 
2000         
68 73 111 55 49
    61 61 77 42
 
3000         
52 50 95 61 60
    62 64 110 59
 
4000         
61 45 74 40 42
    61 51 81 45
 
5000         
50 34 68 45 46
    55 37 77 47
Positive Control Methyl Methane Sulfonate
15         
23 24 163 235 217*
    26 23 156 200
Methyl Methane Sulfonate
250         
41 61 427 347 325*
    52 59 474 302

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium
 0         
109 101 119 36 36
    108 93 101 31
    97 97 130 45
    102 109 98 32
Test Chemical Trisodium ethylenediaminetetraacetate trihydrate (EDTA)
1000         
105 74 137 43 47
    100 78 152 51
 
2000         
82 58 101 41 42
    97 67 126 43
 
3000         
109 62 113 35 44
    99 56 159 54
 
4000         
99 55 106 36 38
    85 45 101 40
 
5000         
77 36 124 54 55
    85 36 145 57
Positive Control 3-Methylcholanthrene
2.5       
72 44 615 286 305*
    70 40 680 323

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium
 0         
60 96 36 20 20
    66 103 25 13
    56 92 48 29
    64 110 35 18
Test Chemical Trisodium ethylenediaminetetraacetate trihydrate (EDTA)
1000         
52 61 41 26 21
    63 75 31 16
 
2000         
67 63 56 28 27
    62 61 48 26
 
3000         
61 40 41 22 31
    71 48 85 40
 
4000         
66 41 56 28 29
    57 35 51 30
 
5000         
70 34 65 31 28
    59 36 45 25
Positive Control 3-Methylcholanthrene
2.5       
34 17 237 236 232*
    34 19 231 229

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination