Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02981-0006-0000-8
Chemical Name:	Trisodium ethylenediaminetetraacetate trihydrate (EDTA)
CASRN:	150-38-9
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	127205
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	64#	92	148.5	78	69
			65	108	133	68
			63	112	132	70
			64	87	113	59
Test Chemical	Trisodium ethylenediaminetetraacetate trihydrate (EDTA)	60	65	107	177	91	83
			74	109	165	74	83
		70	67	113	162	81	68
			58	110	97	56	68
		80	67	100	131	65	59
			63#	116	99	52	59
		90	58	86	119	69	72
			63	93	140	75	72
		100	71	111	142	67	73
			66	101	159	80	73
Positive Control	Methyl Methane Sulfonate	15	49	42	293	198	181*
Positive Control			53	45	261	164	181*

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	84	102	63	25	18
			103	104	57	18
			93	91	50	18
			78	103	24	10
Test Chemical	Trisodium ethylenediaminetetraacetate trihydrate (EDTA)	1000	76	51	45	20	23
			80	42	61	25	23
		2000	87	53	52	20	21
			88	52	61	23	21
		3000	79	38	47	20	21
			77	50	50	22	21
		4000	65	30	49	25	27
			93	32	80	29	27
		5000	76	29	54	24	22
			79	23	49	21	22
Positive Control	Methyl Methane Sulfonate	15	38	18	107	93	93*
Positive Control			52	25	146	93	93*

Nonactivation Trial: 3 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	69	94	88	43	38
			62	109	48	26
			58	87	79	46
			76	110	84	37
Test Chemical	Trisodium ethylenediaminetetraacetate trihydrate (EDTA)	1000	61	59	95	52	57
			55	60	101	61	57
		2000	68	73	111	55	49
			61	61	77	42	49
		3000	52	50	95	61	60
			62	64	110	59	60
		4000	61	45	74	40	42
			61	51	81	45	42
		5000	50	34	68	45	46
			55	37	77	47	46
Positive Control	Methyl Methane Sulfonate	15	23	24	163	235	217*
			26	23	156	200	217*
	Methyl Methane Sulfonate	250	41	61	427	347	325*
			52	59	474	302	325*

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	109	101	119	36	36
			108	93	101	31
			97	97	130	45
			102	109	98	32
Test Chemical	Trisodium ethylenediaminetetraacetate trihydrate (EDTA)	1000	105	74	137	43	47
			100	78	152	51	47
		2000	82	58	101	41	42
			97	67	126	43	42
		3000	109	62	113	35	44
			99	56	159	54	44
		4000	99	55	106	36	38
			85	45	101	40	38
		5000	77	36	124	54	55
			85	36	145	57	55
Positive Control	3-Methylcholanthrene	2.5	72	44	615	286	305*
Positive Control			70	40	680	323	305*

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	60	96	36	20	20
			66	103	25	13
			56	92	48	29
			64	110	35	18
Test Chemical	Trisodium ethylenediaminetetraacetate trihydrate (EDTA)	1000	52	61	41	26	21
			63	75	31	16	21
		2000	67	63	56	28	27
			62	61	48	26	27
		3000	61	40	41	22	31
			71	48	85	40	31
		4000	66	41	56	28	29
			57	35	51	30	29
		5000	70	34	65	31	28
			59	36	45	25	28
Positive Control	3-Methylcholanthrene	2.5	34	17	237	236	232*
Positive Control			34	19	231	229	232*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination