COVID-19 is an emerging, rapidly evolving situation.

Get the latest public health information from CDC and research information from NIH.

U.S. flag

An official website of the United States government

Dot gov

The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Share This:
Study Information
Study Attribute Value
CEBS Accession Number: 002-02981-0001-0000-3
Chemical Name: Trisodium ethylenediaminetetraacetate trihydrate (EDTA)
CASRN: 150-38-9
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 215387_SCE
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Negative 
No Activation  2  Negative 
Induced Rat Liver S9  1_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 09/13/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1044 497 0.476 9.94 26.5 0.000
Test Chemical Trisodium ethylenediaminetetraacetate trihydrate (EDTA) 
5         
 50 1032 516 0.500 10.32 26.5 5.030 
16         
 50 1043 486 0.466 9.72 26.5 -2.120 
50         
 50 1046 487 0.466 9.74 26.5 -2.199 
100         
 50 1041 469 0.451 9.38 26.5 -5.362
Positive Control Mitomycin-C 
 0.001     
 50 1049 711 0.678 14.22 26.5 42.376
Mitomycin-C 
 0.005     
 10 212 406 1.915 40.60 26.5 302.285
Trend: -1.265        
Probability: 0.897        

Trial #: 2    Activation: No Activation    Date: 10/22/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1034 408 0.395 8.16 27.0 0.000
Test Chemical Trisodium ethylenediaminetetraacetate trihydrate (EDTA) 
5         
 50 1040 407 0.391 8.14 27.0 -0.821 
16         
 50 1047 488 0.466 9.76 27.0 18.123 
50         
 50 1040 454 0.437 9.08 27.0 10.633 
100         
 50 1034 438 0.424 8.76 27.0 7.353
Positive Control Mitomycin-C 
 0.001     
 50 1039 630 0.606 12.60 27.0 53.669
Mitomycin-C 
 0.005     
 10 210 273 1.300 27.30 27.0 229.461
Trend: 1.740        
Probability: 0.041        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 09/13/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1048 464 0.443 9.28 26.5 0.000
Test Chemical Trisodium ethylenediaminetetraacetate trihydrate (EDTA) 
5         
 50 1048 516 0.492 10.32 26.5 11.207 
16         
 50 1047 472 0.451 9.44 26.5 1.821 
50         
 50 1049 489 0.466 9.78 26.5 5.287 
100         
 50 1050 486 0.463 9.72 26.5 4.542
Positive Control Cyclophosphamide 
 0.3       
 50 1045 574 0.549 11.48 26.5 24.062
Trend: 0.189        
Probability: 0.425