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Study Information
Study Attribute Value
CEBS Accession Number: 002-02923-0012-0000-1
Chemical Name: p-Anisidine hydrochloride
CASRN: 20265-97-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 802685
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide
 0         
92# 109 223 81 58
    86 126 115 44
    58 64 117 68
    69# 101 83 40
Test Chemical p-Anisidine hydrochloride
50         
66 47 149 75 66
    77 57 143 62
    96 60 174 60
 
100         
79 23 180 76 67
    87 34 151 58
 
200         
64 12 326 170 176*
    66 13 318 160
    51 10 307 199
 
400         
44 7 351 268 252*
    35 7 269 256
    43 7 294 231
 
500         
45 5 343 253 181*
    33 4 109 109
    48# 8 260 182
 
800         
9r 1 114 415 237*
    19 2 128 221
    18# 2 135 252
 
1000         
   
   
Positive Control Methyl Methane Sulfonate
5         
58 63 350 203 233*
    78 71 617 264

Nonactivation Trial: 2 Experiment Call: Rejected
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide
 0         
125r 70 92 25 32
    117 117 106 30
    108 77 95 29
    95 106 103 36
Test Chemical p-Anisidine hydrochloride
100         
77 13 204 89 95*
    36 11 146 135
    74 13 139 62
 
200         
28 4 307 363 294*
    30 5 263 296
    34 6 225 223
 
300         
147*
    48 8 241 167
    36# 6 136 128
 
400         
93*
    65 6 154 79
    53 5 169 106
 
600         
   
 
800         
   
   
Positive Control Methyl Methane Sulfonate
5         
101 70 196 64 162*
    50 10 386 260
    121r 71 292 80

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide
 0         
60 82 80 45 36
    46r 73 59 43
    61 110 63 34
    53 108 46 29
Test Chemical p-Anisidine hydrochloride
12.5       
64 83 142 74 62*
    81 134 115 48
    80 150 155 65
 
25         
66 58 66 33 64*
    72 58 148 68
    68 59 186 91
 
50         
54 30 92 57 61*
    56 37 123 73
    49 38 76 52
 
100         
35 13 197 189 145*
    54 16 173 106
    34 11 145 141
 
200         
32 6 176 185 220*
    29# 6 168 193
    33 6 276 280
 
400         
37 6 274 249 201*
    37 6 168 153
Positive Control Methyl Methane Sulfonate
5         
66 55 508 259 208*
    48 47 228 157

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination