Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02722-0006-0000-1
Chemical Name:	Potassium chloride
CASRN:	7447-40-7
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	012218
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	86	97	53	21	26
			68	82	65	32
			68	91	49	24
			82	130	67	27
Test Chemical	Potassium chloride	156	108	128	49	15	18
			104	122	68	22	18
		313	89	116	60	22	26
			83#	116	72	29	26
		625	86	119	51	20	18
			100	133	48	16	18
		1250	103	120	55	18	18
			91	139	49	18	18
		2500	95	123	59	21	25
			66	95	57	29	25
		5000	71	50	81	38	28
			93	79	52	19	28
Positive Control	Ethyl Methane Sulfonate	500	29	14	696	786	862*
			27	21	789	962
			21	16	535	836

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	56	87	30	18	20
			80#	120	64.5	27
			55	83	29	18
			69	110	33	16
Test Chemical	Potassium chloride	500	45	56	26	19	16
			62	75	28	15
			58	73	24	14
		1000	54	87	24	15	15
			64	100	34	18
			52	98	19	12
		2000	63	85	32	17	16
			75	83	38	17
			81	82	34	14
		4000	72	59	51	24	18
			63	64	25	13
			63	61	32	17
		5000	65	56	31	16	18
			58	40	37	21
			61	53	32	18
Positive Control	Ethyl Methane Sulfonate	500	65	47	531	272	289*
			50	21	428	287
			58	51	532	308

Nonactivation Trial: 3 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	100	91	164	54	52
Vehicle Control			105	109	153	49	52
Test Chemical	Potassium chloride	4000	97	77	123	42	44
			90	70	104	39
			84	60	130	52
		5000	72#	45	146	68	61
			76#	57	158	69
			107	63	146	46
		7000	84#	33	312	124	133*
			86	26	365	141	133*
		8000	79	12	494	209	199*
			85	11	481	189	199*
		9000


Positive Control	Ethyl Methane Sulfonate	250	55#	20	962	587	521*
			62#	32	941	503
			71	32	1011	475

Nonactivation Trial: 4 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	98	91	102	35	34
Vehicle Control			102	109	104	34	34
Test Chemical	Potassium chloride	4000	83	61	79	32	28
			88	70	60	23
			96	77	82	29
		5000	67#	44	86	43	40
			75#	58	106	47
			105	63	93	30
		7000	85	26	208	81	76*
			85#	34	182	72	76*
		8000	78	13	282	121	115*
			83	11	271	109	115*
		9000


Positive Control	Ethyl Methane Sulfonate	250	49#	18	754	518	472*
			58#	30	787	456
			64	29	847	441

Non-Induced S9 Trial: 1 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	88	103	97	37	44
			89	85	172	64
			101	126	104	34
			109	85	128	39
Test Chemical	Potassium chloride	500	110	140	96	29	33
			93	96	90	32
			82	104	92	38
		1000	97	97	121	42	38
			109	105	125	38
			110	117	113	34
		2000	103	84	122	39	46
			92	61	152	55
			99	76	129	44
		4000	74	24	166	74	73*
			91	28	194	71
			86	27	187	72
		5000	84	16	190	75	69*
			94#	17	219	78
			88	18	146	55
Positive Control	3-Methylcholanthrene	7	68	45	406	200	191*
Positive Control			73#	44	397.5	183	191*

Non-Induced S9 Trial: 2 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	104	97	91	29	26
			124r	97	77	21
			119	96	116	32
			89	107	41	15
Test Chemical	Potassium chloride	500	97	88	78	27	28
			81	62	78	32
			97	71	68	23
		1000	75	72	50	22	21
			106#	87	73.5	23
			92#	83	45	16
		2000	109#	74	86	26	29
			96	60	85	29
			84	67	77	30
		4000	85	33	83	32	39
			84	46	115	46
			92	26	105	38
		5000	104	45	93	30	38
			101	28	151	50
			110	24	111	34
Positive Control	3-Methylcholanthrene	7	51	47	291	192	154*
			81	68	305	125
			67	45	290	145

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	74	101	47	21	24
			91	109	39	14
			66	110	61	31
			62	80	55	29
Test Chemical	Potassium chloride	156	95	132	56	20	26
			71	104	67	31	26
		313	93	114	66	24	22
			98	121	58	20	22
		625	90	107	74	27	27
			83	87	66	27	27
		1250	96	114	70	24	22
			86	101	53	21	22
		2500	70	59	83	40	38
			75	87	80	36	38
		5000	74	33	116	53	43*
			83	39	83	33	43*
Positive Control	3-Methylcholanthrene	5	25	10	280	371	408*
			23	7	352	510
			32	14	330	344

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	95	100	90	31	28
			95	100	75	26
			89	98	75	28
			104	102	76	24
Test Chemical	Potassium chloride	500	78	86	77	33	35
			96	90	114	40
			83	119	82	33
		1000	89	80	85	32	25
			86	79	49	19
			77	79	58	25
		2000	81	102	69	28	29
			97	87	91	31
			97	75	80	28
		4000	69	28	67	33	39
			85	28	117	46
			108	37	125	39
		5000	77	25	161	70	54*
			90	43	100	37
			71	36	116	54
Positive Control	3-Methylcholanthrene	5	52	19	572	365	361*
			56	18	584	351
			54	15	593	367

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	133r	111	85	21	13
			118	110	46	13
			115#	102	54	16
			97	88	30	10
Test Chemical	Potassium chloride	2	85	65	33	13	14
			87#	72	30	11
			79#	74	42	18
		4	97	53	49	17	17
			96	53	68	24
			87#	59	30	12
		5	101	49	85	28	22*
			96	38	63	22
			103	51	50	16
		6	103#	34	82.5	27	27*
			174r	68	83	16
			107	26	90	28
		7	99#	17	94.5	32	40*
			87	14	109	42
			94	14	131	47
		8

		10


Positive Control	3-Methylcholanthrene	2.5	105	55	472	149	135*
			109	55	442	135
			106#	58	385.5	122

Induced S9 Trial: 4 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	110	88	190	58	63
			101	97	207	69
			110	115	209	63
			131r	114	251	64
Test Chemical	Potassium chloride	2	101	65	158	52	69
			82	61	183	75
			87	49	208	80
		4	87	19	326	125	105*
			74	22	149	68
			69	23	253	123
		5	77	23	207	90	88
			99	18	269	90
			85	18	210	83
		6	80	8	243	102	95*
			64#	10	180	93
			82	9	219	89
		7

			80#	7	180	75
		8



Positive Control	3-Methylcholanthrene	2.5	90	52	614	228	221*
			78#	46	534	228
			84	68	522	208

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination