COVID-19 is an emerging, rapidly evolving situation.

Get the latest public health information from CDC and research information from NIH.

U.S. flag

An official website of the United States government

Dot gov

The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Share This:
Study Information
Study Attribute Value
CEBS Accession Number: 002-02500-0001-0000-0
Chemical Name: N,N'-Di-2-napthyl-p-phenylenediamine
CASRN: 93-46-9
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 999918_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
No Activation  2  Negative 
No Activation  3  Positive 
Induced Rat Liver S9  1_S9  Questionable 
Induced Rat Liver S9  2_S9  Questionable 

Trial #: 1    Activation: No Activation    Date: 11/19/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1050 460 0.438 9.20 26.0 0.000
Test Chemical N,N'-Di-2-napthyl-p-phenylenediamine 
16         
 50 1050 492 0.469 9.84 26.0 6.957 
50         
 50 1049 525 0.500 10.50 26.0 14.239 
160         
 50 1048 596 0.569 11.92 26.0 29.812 
500         
 50 1049 565 0.539 11.30 26.0 22.943 
1600         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.0E-4    
 50 1050 605 0.576 12.10 26.0 31.522
Mitomycin-C 
 0.005     
 10 210 313 1.490 31.30 26.0 240.217
Trend: 4.354        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 01/07/1986    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1047 437 0.417 8.74 26.5 0.000
Test Chemical N,N'-Di-2-napthyl-p-phenylenediamine 
100         
 50 1047 438 0.418 8.76 26.5 0.229 
200         
 50 1043 463 0.444 9.26 26.5 6.356 
400         
 50 1042 466 0.447 9.32 26.5 7.148 
600         
 50 1046 448 0.428 8.96 26.5 2.615 
800         
 0 0 0 0.000 0.00 26.5 N/A
Positive Control Mitomycin-C 
 0.0E-4    
 50 1047 544 0.520 10.88 26.5 24.485
Mitomycin-C 
 0.005     
 10 209 264 1.263 26.40 26.5 202.638
Trend: 0.867        
Probability: 0.193        

Trial #: 3    Activation: No Activation    Date: 07/01/1986    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1050 391 0.372 7.82 26.0 0.000
Test Chemical N,N'-Di-2-napthyl-p-phenylenediamine 
50         
 50 1047 460 0.439 9.20 26.0 17.984 
160         
 50 1049 493 0.470 9.86 26.0 26.207 
500         
 50 1050 562 0.535 11.24 26.0 43.734
Positive Control Mitomycin-C 
 0.0E-4    
 50 1049 537 0.512 10.74 26.0 37.471
Mitomycin-C 
 0.005     
 10 209 286 1.368 28.60 26.0 267.479
Trend: 5.579        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 11/19/1985    Trial Call: Questionable   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1046 457 0.437 9.14 26.0 0.000
Test Chemical N,N'-Di-2-napthyl-p-phenylenediamine 
16         
 50 1049 477 0.455 9.54 26.0 4.078 
50         
 50 1050 491 0.468 9.82 26.0 7.031 
160         
 50 1047 495 0.473 9.90 26.0 8.212 
500         
 50 1053 552 0.524 11.04 26.0 19.985
Positive Control Cyclophosphamide 
 0.15      
 50 1049 960 0.915 19.20 26.0 109.465
Cyclophosphamide 
 0.6       
 10 210 300 1.429 30.00 26.0 226.977
Trend: 2.876        
Probability: 0.002        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 07/01/1986    Trial Call: Questionable   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1044 464 0.444 9.28 26.0 0.000
Test Chemical N,N'-Di-2-napthyl-p-phenylenediamine 
50         
 50 1044 559 0.535 11.18 26.0 20.474 
160         
 50 1045 484 0.463 9.68 26.0 4.211 
500         
 50 1047 551 0.526 11.02 26.0 18.410
Positive Control Cyclophosphamide 
 0.15      
 50 1047 581 0.555 11.62 26.0 24.857
Cyclophosphamide 
 0.6       
 10 210 240 1.143 24.00 26.0 157.143
Trend: 1.777        
Probability: 0.038