Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02476-0010-0000-2
Chemical Name:	Michler's ketone
CASRN:	90-94-8
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G29620
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	84	131	46	18	26
			71	87	68	32
			63	68	62	33
			90	115	56	21
Test Chemical	Michler's ketone	6	83	81	47	19	22
			78	87	60	26	22
		7	74	69	57	26	24
			92	64	64	23	24
		8	77	57	88	38	35
			81	73	79	33	35
		9	80	70	81	34	33
			78	55	73	31	33
		10	63	51	111	59	55*
			74	55	113	51	55*
		12	68	39	140	69	67*
			82#	45	163.5	66	67*
Positive Control	Ethyl Methane Sulfonate	500	41	31	675	547	547*
			34	28	618	603
			44	39	647	490

Nonactivation Trial: 2 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	89	96	44	17	16
			98	121	37	13
			74	80	49	22
			83	104	36	14
Test Chemical	Michler's ketone	1.25	109	64	79	24	26*
			106	87	80	25
			82	51	67	27
		2.5	76	59	50	22	18
			87	57	41	16
			85	81	45	18
		5	91	76	63	23	21
			100	79	52	17
			93	61	62	22
		7.5	68	49	38	19	20
			83	66	52	21
			101	53	60	20
		10	74	46	50	23	21
			82	58	53	22
			92	85	54	20
		12					22
			72	37	47	22
			76	59	52	23
		15


Positive Control	Ethyl Methane Sulfonate	500	52	36	743	472	558*
			41	31	606	497
			36	22	761	705

Nonactivation Trial: 3 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	86	111	52	20	17
			96	102	61	21
			89#	92	42	16
			78	95	24	10
Test Chemical	Michler's ketone	2	90	48	48	18
		4	45	31	51	38	29*
			82	36	63	26
			80	37	53	22
		8	74	49	48	22	25
			46	24	34	25
			40	22	36	30
		10	51	26	49	32	37*
			50	27	38	25
			49	22	80	55
		12	58	34	35	20	22
			55	24	56	34
			70	25	24	11
		14	65	26	63	32	35*
			51	19	61	40
			57	27	57	33
Positive Control	Ethyl Methane Sulfonate	500	8r	1	261	1088	1020*
Positive Control			12	3	362	1020	1020*

Nonactivation Trial: 4 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	72	97	35	16	21
			61	89	53	29
			88	119	51	19
			76	94	43	19
Test Chemical	Michler's ketone	4	56#	44	33	20	19
			49	45	33	23
			52	34	24	15
		8	53	40	22	14	16
			48	36	15	11
			35	33	26	25
		10	50#	29	38	25	24
			62	39	47	25
			58	36	36	21
		12	46	35	26	19	21
			51	33	36	24	21
		14	61	33	21	12	19
			48	30	31	22
			64	39	43	22
		16	53	43	34	21	23
			64	39	47	25	23
Positive Control	Ethyl Methane Sulfonate	500	16	9	678	1413	1226*
			17	10	681	1310
			30#	25	850.5	956

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	103	106	91	29	30
			84#	100	85.5	34
			79	80	81	34
			78	114	56	24
Test Chemical	Michler's ketone	0.5	92	107	44	16	23
			92	76	84	30	23
		1	98#	26	132	45	47
			77	65	111	48	47
		2	94	48	113	40	41
			88	47	108	41	41
		4	77	46	159	69	59*
			98	51	147	50	59*
		8	77	15	294	127	120*
			88	28	298	112	120*
		16

Positive Control	3-Methylcholanthrene	5	43	18	380	293	272*
Positive Control			60	29	448	250	272*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	114	110	98	29	34
			101	109	112	37
			71	66	87	41
			113	116	100	30
Test Chemical	Michler's ketone	4	104	81	246	79	82*
			70	61	179	85	82*
		6	71	52	306	145	126*
			97	58	313	108	126*
		8	83	35	377	152	168*
			73	22	406	185	168*
		10	68	15	495	243	243*
			58	19	428	244	243*
		12	49	9	534	365	360*
			49	9	523	355	360*
		15	38	4	422	369	375*
			36	4	406	381	375*
Positive Control	3-Methylcholanthrene	5	45	27	496	369	328*
			54	24	540	334
			66	37	558	280

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination