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Study Information
Study Attribute Value
CEBS Accession Number: 002-02465-0002-0000-1
Chemical Name: Methylene chloride
CASRN: 75-09-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 676332_CA
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  Negative 
Induced Rat Liver S9  1_S9  Negative 

Trial #:1   Activation: No Activation    Date: 06/14/1988    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL
Total
Cells Examined
Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
Vehicle Control: Negative (Not Specified)
0         
200 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: Methylene chloride
1600         
200 1 0.005 0.5 0 0.000 0.0 1 0.005 0.5 0 0.000 0.0
3000         
200 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
5000         
200 1 0.005 0.5 0 0.000 0.0 1 0.005 0.5 0 0.000 0.0
Positive Control: Mitomycin-C
 0.0625    
200 23 0.115 11.0 10 0.050 5.0 13 0.065 6.0 0 0.000 0.0
Mitomycin-C
 0.25      
50 14 0.280 26.0 9 0.180 18.0 5 0.100 10.0 0 0.000 0.0
Trend: 0.594 0.000 0.594  
Probability: 0.276 0.000 0.276  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 06/14/1988    Harvest Time: 13 hour(s)   Trial Call: Negative   
  Dose
µg/mL
Total
Cells Examined
Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
Vehicle Control: Negative (Not Specified)
0         
200 2 0.010 1.0 1 0.005 0.5 1 0.005 0.5 0 0.000 0.0
Test Chemical: Methylene chloride
1600         
200 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
3000         
200 1 0.005 0.5 0 0.000 0.0 1 0.005 0.5 0 0.000 0.0
5000         
200 1 0.005 0.5 0 0.000 0.0 1 0.005 0.5 0 0.000 0.0
Positive Control: Cyclophosphamide
2.5       
200 24 0.120 10.5 2 0.010 1.0 22 0.110 9.5 0 0.000 0.0
Cyclophosphamide
7.5       
50 17 0.340 30.0 5 0.100 10.0 12 0.240 24.0 0 0.000 0.0
Trend: -0.443 -1.351 0.269  
Probability: 0.671 0.912 0.394