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Study Information
Study Attribute Value
CEBS Accession Number: 002-02465-0001-0000-0
Chemical Name: Methylene chloride
CASRN: 75-09-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 676332_SCE
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 05/10/1988    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1045 381 0.365 7.62 26.0 0.000
Test Chemical Methylene chloride 
160         
 50 1038 432 0.416 8.64 26.0 14.150 
500         
 50 1043 411 0.394 8.22 26.0 8.081 
1600         
 50 1033 419 0.406 8.38 26.0 11.251 
5000         
 50 1040 425 0.409 8.50 26.0 12.085
Positive Control Mitomycin-C 
 0.0E-4    
 50 1030 556 0.540 11.12 26.0 48.057
Mitomycin-C 
 0.005     
 10 208 296 1.423 29.60 26.0 290.319
Trend: 1.259        
Probability: 0.104        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 05/10/1988    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1046 361 0.345 7.22 26.0 0.000
Test Chemical Methylene chloride 
160         
 50 1049 353 0.337 7.06 26.0 -2.496 
500         
 50 1050 368 0.350 7.36 26.0 1.551 
1600         
 50 1045 387 0.370 7.74 26.0 7.305 
5000         
 50 1048 376 0.359 7.52 26.0 3.956
Positive Control Cyclophosphamide 
 0.1       
 50 1048 500 0.477 10.00 26.0 38.240
Cyclophosphamide 
 0.6       
 10 210 196 0.933 19.60 26.0 170.434
Trend: 1.056        
Probability: 0.146