COVID-19 is an emerging, rapidly evolving situation.

Get the latest public health information from CDC and research information from NIH.

U.S. flag

An official website of the United States government

Dot gov

The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Share This:
Study Information
Study Attribute Value
CEBS Accession Number: 002-01931-0002-0000-8
Chemical Name: Cyclophosphamide monohydrate
CASRN: 6055-19-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 661949_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  Negative 
Induced Rat Liver S9  1_S9  Positive 
Induced Rat Liver S9  2_S9  Positive 

Trial #:1   Activation: No Activation    Date: 11/03/1988    Harvest Time: 15.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL
Total
Cells Examined
Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide
0         
200 6 0.030 2.0 1 0.005 0.5 5 0.025 1.5 0 0.000 0.0
Test Chemical: Cyclophosphamide monohydrate
1081         
200 2 0.010 1.0 0 0.000 0.0 2 0.010 1.0 0 0.000 0.0
2325         
200 14 0.070 2.0 2 0.010 1.0 2 0.010 1.0 10 0.050 1.0
5000         
200 2 0.010 1.0 1 0.005 0.5 1 0.005 0.5 0 0.000 0.0
Positive Control: Mitomycin-C
 0.1       
200 39 0.195 15.0 21 0.105 9.0 18 0.090 7.0 0 0.000 0.0
Mitomycin-C
 0.4       
50 13 0.260 26.0 10 0.200 20.0 3 0.060 6.0 0 0.000 0.0
Trend: -0.520 0.448 -0.953  
Probability: 0.699 0.327 0.830  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 11/03/1988    Harvest Time: 16.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL
Total
Cells Examined
Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide
0         
100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: Cyclophosphamide monohydrate
15         
50 58 1.160 54.0 25 0.500 42.0 13 0.260 22.0 20 0.400 2.0
32         
50 96 1.920 66.0 49 0.980 58.0 26 0.520 36.0 21 0.420 3.0
70         
50 116 2.320 88.0 64 1.280 68.0 52 1.040 64.0 0 0.000 0.0
Positive Control: Cyclophosphamide
5         
200 42 0.210 19.0 22 0.110 10.5 20 0.100 9.5 0 0.000 0.0
Cyclophosphamide
15         
50 25 0.500 38.0 16 0.320 28.0 9 0.180 16.0 0 0.000 0.0
Trend: 10.877 9.203 8.620  
Probability: 0.000 0.000 0.000  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 11/14/1988    Harvest Time: 16.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL
Total
Cells Examined
Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide
0         
100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: Cyclophosphamide monohydrate
32         
25 53 2.120 76.0 22 0.880 56.0 11 0.440 32.0 20 0.800 2.0
70         
25 62 2.480 96.0 27 1.080 68.0 24 0.960 76.0 11 0.440 2.0
150         
25 101 4.040 88.0 42 1.680 60.0 18 0.720 44.0 41 1.640 5.0
Positive Control: Cyclophosphamide
5         
200 47 0.235 18.0 21 0.105 10.0 26 0.130 9.0 0 0.000 0.0
Cyclophosphamide
15         
50 29 0.580 40.0 20 0.400 26.0 9 0.180 18.0 0 0.000 0.0
Trend: 10.606 8.186 7.375  
Probability: 0.000 0.000 0.000