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Study Information
Study Attribute Value
CEBS Accession Number: 002-01931-0001-0000-7
Chemical Name: Cyclophosphamide monohydrate
CASRN: 6055-19-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 661949_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
No Activation  2  Positive 
Induced Rat Liver S9  1_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 10/28/1988    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1042 398 0.382 7.96 26.0 0.000
Test Chemical Cyclophosphamide monohydrate 
50         
 50 1043 427 0.409 8.54 26.0 7.184 
167         
 50 1040 559 0.538 11.18 26.0 40.722 
500         
 50 1044 716 0.686 14.32 26.0 79.555 
1667         
 50 1045 1060 1.014 21.20 26.0 165.567
Positive Control Mitomycin-C 
 0.001     
 50 1045 714 0.683 14.28 26.0 78.882
Mitomycin-C 
 0.005     
 10 208 303 1.457 30.30 26.0 281.385
Trend: 20.229        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 11/10/1988    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1039 381 0.367 7.62 26.0 0.000
Test Chemical Cyclophosphamide monohydrate 
50         
 50 1045 379 0.363 7.58 26.0 -1.096 
167         
 50 1032 480 0.465 9.60 26.0 26.839 
500         
 50 1048 721 0.688 14.42 26.0 87.614 
1667         
 50 1035 1238 1.196 24.76 26.0 226.190
Positive Control Mitomycin-C 
 0.001     
 50 1044 650 0.623 13.00 26.0 69.787
Mitomycin-C 
 0.005     
 10 211 344 1.630 34.40 26.0 344.597
Trend: 25.739        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 10/28/1988    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1045 401 0.384 8.02 26.0 0.000
Test Chemical Cyclophosphamide monohydrate 
 0.5       
 50 1045 934 0.894 18.68 26.0 132.918 
1.7       
 50 1050 2423 2.308 48.46 26.0 501.362 
5         
 50 1049 4029 3.841 80.58 26.0 900.907 
17         
 50 1044 6932 6.640 138.64 31.0 1630.334
Positive Control Cyclophosphamide 
 0.125     
 50 1039 636 0.612 12.72 26.0 59.519
Cyclophosphamide 
 0.5       
 10 210 350 1.667 35.00 26.0 334.331
Trend: 94.196        
Probability: 0.000