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Study Information
Study Attribute Value
CEBS Accession Number: 002-01849-0002-0000-6
Chemical Name: Chlorambucil
CASRN: 305-03-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 586147_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  Positive 
Induced Rat Liver S9  1_S9  Positive 
No Activation  Positive 

Trial #:1   Activation: No Activation    Date: 11/14/1984    Harvest Time: 10.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL
Total
Cells Examined
Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
Vehicle Control: Negative (Not Specified)
0         
50 2 0.040 4.0 1 0.020 2.0 1 0.020 2.0 0 0.000 0.0
Dimethyl Sulfoxide
0         
50 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: Chlorambucil
50.2       
10 56 5.600 100.0 4 0.400 20.0 11 1.100 60.0 41 4.100 50.0
100.5       
10 10 1.000 100.0 0 0.000 0.0 0 0.000 0.0 10 1.000 100.0
150         
10 10 1.000 100.0 0 0.000 0.0 0 0.000 0.0 10 1.000 100.0
Positive Control: Mitomycin-C
 0.15      
50 9 0.180 14.0 4 0.080 8.0 5 0.100 6.0 0 0.000 0.0
Mitomycin-C
 0.5       
25 11 0.440 44.0 6 0.240 24.0 5 0.200 20.0 0 0.000 0.0
Trend: 7.999 0.274 0.487  
Probability: 0.000 0.392 0.313  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 10/30/1984    Harvest Time: 20.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL
Total
Cells Examined
Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
Vehicle Control: Negative (Not Specified)
0         
100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Dimethyl Sulfoxide
0         
100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: Chlorambucil
12.6       
10 36 3.600 100.0 17 1.700 90.0 9 0.900 50.0 10 1.000 10.0
25.2       
10 64 6.400 100.0 8 0.800 40.0 16 1.600 50.0 40 4.000 40.0
50.3       
10 100 10.000 100.0 0 0.000 0.0 0 0.000 0.0 100 10.000 100.0
100.5       
0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide
6.25      
100 16 0.160 15.0 5 0.050 5.0 11 0.110 11.0 0 0.000 0.0
Cyclophosphamide
12.5       
25 18 0.720 48.0 1 0.040 4.0 17 0.680 44.0 0 0.000 0.0
Trend: 10.118 3.461 3.366  
Probability: 0.000 0.000 0.000  


Trial #:2   Activation: No Activation    Date: 10/31/1984    Harvest Time: 10.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL
Total
Cells Examined
Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
Vehicle Control: Negative (Not Specified)
0         
100 4 0.040 4.0 1 0.010 1.0 3 0.030 3.0 0 0.000 0.0
Dimethyl Sulfoxide
0         
100 4 0.040 4.0 0 0.000 0.0 4 0.040 4.0 0 0.000 0.0
Test Chemical: Chlorambucil
20         
25 30 1.200 56.0 8 0.320 24.0 12 0.480 40.0 10 0.400 4.0
25         
25 36 1.440 56.0 11 0.440 40.0 5 0.200 20.0 20 0.800 8.0
50         
10 58 5.800 90.0 4 0.400 30.0 4 0.400 30.0 50 5.000 5.0
Positive Control: Mitomycin-C
 0.15      
100 43 0.430 24.0 17 0.170 15.0 16 0.160 12.0 10 0.100 1.0
Mitomycin-C
 0.5       
25 22 0.880 48.0 14 0.560 36.0 8 0.320 24.0 0 0.000 0.0
Trend: 8.083 5.404 3.691  
Probability: 0.000 0.000 0.000