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Study Information
Study Attribute Value
CEBS Accession Number: 002-01849-0002-0000-6
Chemical Name: Chlorambucil
CASRN: 305-03-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 586147_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
Induced Rat Liver S9  1_S9  Positive 
No Activation  2  Positive 

Trial #:1   Activation: No Activation    Date: 11/14/1984    Harvest Time: 10.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 50 2 0.040 4.0 1 0.020 2.0 1 0.020 2.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 50 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: Chlorambucil 
50.2       
 10 56 5.600 100.0 4 0.400 20.0 11 1.100 60.0 41 4.100 50.0 
100.5       
 10 10 1.000 100.0 0 0.000 0.0 0 0.000 0.0 10 1.000 100.0 
150         
 10 10 1.000 100.0 0 0.000 0.0 0 0.000 0.0 10 1.000 100.0
Positive Control: Mitomycin-C 
 0.15      
 50 9 0.180 14.0 4 0.080 8.0 5 0.100 6.0 0 0.000 0.0
Mitomycin-C 
 0.5       
 25 11 0.440 44.0 6 0.240 24.0 5 0.200 20.0 0 0.000 0.0
Trend: 7.999 0.274 0.487  
Probability: 0.000 0.392 0.313  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 10/30/1984    Harvest Time: 20.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: Chlorambucil 
12.6       
 10 36 3.600 100.0 17 1.700 90.0 9 0.900 50.0 10 1.000 10.0 
25.2       
 10 64 6.400 100.0 8 0.800 40.0 16 1.600 50.0 40 4.000 40.0 
50.3       
 10 100 10.000 100.0 0 0.000 0.0 0 0.000 0.0 100 10.000 100.0 
100.5       
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
6.25      
 100 16 0.160 15.0 5 0.050 5.0 11 0.110 11.0 0 0.000 0.0
Cyclophosphamide 
12.5       
 25 18 0.720 48.0 1 0.040 4.0 17 0.680 44.0 0 0.000 0.0
Trend: 10.118 3.461 3.366  
Probability: 0.000 0.000 0.000  


Trial #:2   Activation: No Activation    Date: 10/31/1984    Harvest Time: 10.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 4 0.040 4.0 1 0.010 1.0 3 0.030 3.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 4 0.040 4.0 0 0.000 0.0 4 0.040 4.0 0 0.000 0.0
Test Chemical: Chlorambucil 
20         
 25 30 1.200 56.0 8 0.320 24.0 12 0.480 40.0 10 0.400 4.0 
25         
 25 36 1.440 56.0 11 0.440 40.0 5 0.200 20.0 20 0.800 8.0 
50         
 10 58 5.800 90.0 4 0.400 30.0 4 0.400 30.0 50 5.000 5.0
Positive Control: Mitomycin-C 
 0.15      
 100 43 0.430 24.0 17 0.170 15.0 16 0.160 12.0 10 0.100 1.0
Mitomycin-C 
 0.5       
 25 22 0.880 48.0 14 0.560 36.0 8 0.320 24.0 0 0.000 0.0
Trend: 8.083 5.404 3.691  
Probability: 0.000 0.000 0.000