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Study Information
Study Attribute Value
CEBS Accession Number: 002-01849-0001-0000-5
Chemical Name: Chlorambucil
CASRN: 305-03-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 586147_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  Positive 
No Activation  Positive 
Induced Rat Liver S9  1_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 10/04/1984    Trial Call: Positive   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide
0         
50 1049 412 0.393 8.24 26.5 0.000
Test Chemical Chlorambucil
 0.2       
5 104 383 3.683 76.60 26.5 837.656
 0.5       
5 105 613 5.838 122.60 26.5 1386.447
1.7       
5 104 573 5.510 114.60 36.0 1302.812
5         
0 0 0 0.000 0.00 36.0 N/A
Positive Control Mitomycin-C
 0.001     
50 1049 564 0.538 11.28 26.5 36.893
Mitomycin-C
 0.01      
5 104 245 2.356 49.00 26.5 499.806
Trend: 58.065        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 10/24/1984    Trial Call: Positive   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide
0         
25 525 219 0.417 8.76 25.5 0.000
Test Chemical Chlorambucil
3         
5 105 419 3.990 83.80 35.0 856.621
4         
5 104 596 5.731 119.20 35.0 1273.815
5         
5 105 614 5.848 122.80 35.0 1301.826
Positive Control Mitomycin-C
 0.001     
25 525 326 0.621 13.04 25.5 48.858
Mitomycin-C
 0.01      
5 105 236 2.248 47.20 25.5 438.813
Trend: 44.421        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 10/04/1984    Trial Call: Positive   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide
0         
50 1049 437 0.417 8.74 26.5 0.000
Test Chemical Chlorambucil
 0.5       
5 104 514 4.942 102.80 26.5 1086.380
1.7       
5 104 549 5.279 109.80 36.0 1167.165
5         
0 0 0 0.000 0.00 36.0 N/A
16.7       
5 105 602 5.733 120.40 36.0 1276.262
50         
0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide
 0.4       
50 1049 547 0.521 10.94 26.5 25.172
Cyclophosphamide
2         
5 105 154 1.467 30.80 26.5 252.067
Trend: 54.780        
Probability: 0.000