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Study Information
Study Attribute Value
CEBS Accession Number: 002-01849-0001-0000-5
Chemical Name: Chlorambucil
CASRN: 305-03-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 586147_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
No Activation  2  Positive 
Induced Rat Liver S9  1_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 10/04/1984    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1049 412 0.393 8.24 26.5 0.000
Test Chemical Chlorambucil 
 0.2       
 5 104 383 3.683 76.60 26.5 837.656 
 0.5       
 5 105 613 5.838 122.60 26.5 1386.447 
1.7       
 5 104 573 5.510 114.60 36.0 1302.812 
5         
 0 0 0 0.000 0.00 36.0 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1049 564 0.538 11.28 26.5 36.893
Mitomycin-C 
 0.01      
 5 104 245 2.356 49.00 26.5 499.806
Trend: 58.065        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 10/24/1984    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 25 525 219 0.417 8.76 25.5 0.000
Test Chemical Chlorambucil 
3         
 5 105 419 3.990 83.80 35.0 856.621 
4         
 5 104 596 5.731 119.20 35.0 1273.815 
5         
 5 105 614 5.848 122.80 35.0 1301.826
Positive Control Mitomycin-C 
 0.001     
 25 525 326 0.621 13.04 25.5 48.858
Mitomycin-C 
 0.01      
 5 105 236 2.248 47.20 25.5 438.813
Trend: 44.421        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 10/04/1984    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1049 437 0.417 8.74 26.5 0.000
Test Chemical Chlorambucil 
 0.5       
 5 104 514 4.942 102.80 26.5 1086.380 
1.7       
 5 104 549 5.279 109.80 36.0 1167.165 
5         
 0 0 0 0.000 0.00 36.0 N/A 
16.7       
 5 105 602 5.733 120.40 36.0 1276.262 
50         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
 0.4       
 50 1049 547 0.521 10.94 26.5 25.172
Cyclophosphamide 
2         
 5 105 154 1.467 30.80 26.5 252.067
Trend: 54.780        
Probability: 0.000