Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01703-0008-0000-1
Chemical Name:	Benzofuran
CASRN:	271-89-6
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	158938
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	103	99	197	64	79
			63	96	155	82
			94	104	177	63
			86	101	272	106
Test Chemical	Benzofuran	12.5	100	131	157	52	56
			91	137	162	59	56
		25	83	110	243	97	80
			82	164	153	62	80
		50	85#	138	197	77	76
			97	123	217	74	76
		100	88	61	163	62	56
			82	86	123	50	56
		200	125r	17	436	117
			90	14	383	142
Positive Control	Methyl Methane Sulfonate	15	58	52	443	257	267*
Positive Control			42	34	352	277	267*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	92	100	152	55	58
			89	102	130	49
			84	105	158	63
			108	93	214	66
Test Chemical	Benzofuran	50	72	66	136	63	58
			93	68	146	52	58
		100	68#	31	180	88	89
			65	26	175	90	89
		150	32#	6	267	280	229*
			45	10	242	179	229*
		200

Positive Control	Methyl Methane Sulfonate	15	42	24	469	371	337*
Positive Control			41	26	368	303	337*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	109	101	92	28	31
			94	99	81	29
			93	92	86	31
			99	108	104	35
Test Chemical	Benzofuran	100	107	33	203	63	58*
			75	30	117	52	58*
		125	73	21	177	81	75*
			59	22	122	69	75*
		150	67	16	218	109	103*
			82	11	241	98	103*
		175	78	10	335	143	134*
			84	9	315	125	134*
		200	89	8	364	136	136*
			72	9	291	136	136*
		225

Positive Control	Methyl Methane Sulfonate	15	27	16	254	314	330*
Positive Control			31	19	323	345	330*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination