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Study Information
Study Attribute Value
CEBS Accession Number: 002-01678-0002-0000-6
Chemical Name: Azodicarbonamide
CASRN: 123-77-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 998177_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  Negative 
Induced Rat Liver S9  1_S9  Positive 
No Activation  Questionable 
Induced Rat Liver S9  2_S9  Weakly Positive* 

Trial #:1   Activation: No Activation    Date: 04/05/1984    Harvest Time: 26.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL
Total
Cells Examined
Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
Vehicle Control: Negative (Not Specified)
0         
100 3 0.030 3.0 0 0.000 0.0 3 0.030 3.0 0 0.000 0.0
Dimethyl Sulfoxide
0         
100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
Test Chemical: Azodicarbonamide
402         
100 3 0.030 3.0 2 0.020 2.0 1 0.010 1.0 0 0.000 0.0
498         
100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0
600         
100 3 0.030 3.0 0 0.000 0.0 2 0.020 2.0 1 0.010 1.0
720         
0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C
 0.025     
100 10 0.100 7.0 4 0.040 3.0 6 0.060 5.0 0 0.000 0.0
Mitomycin-C
 0.0625    
25 12 0.480 32.0 5 0.200 20.0 7 0.280 16.0 0 0.000 0.0
Trend: 0.273 -0.262 -0.007  
Probability: 0.393 0.603 0.503  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 04/05/1984    Harvest Time: 18.7 hour(s)   Trial Call: Positive   
  Dose
µg/mL
Total
Cells Examined
Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
Vehicle Control: Negative (Not Specified)
0         
100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
Dimethyl Sulfoxide
0         
100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: Azodicarbonamide
900         
200 17 0.085 6.5 8 0.040 3.0 9 0.045 3.5 0 0.000 0.0
1020         
200 9 0.045 4.5 3 0.015 1.5 6 0.030 3.0 0 0.000 0.0
1104         
200 13 0.065 6.0 7 0.035 3.5 6 0.030 2.5 0 0.000 0.0
1290         
0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide
2.5       
100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0
Cyclophosphamide
12.5       
25 14 0.560 40.0 6 0.240 24.0 8 0.320 20.0 0 0.000 0.0
Trend: 1.508 1.296 0.825  
Probability: 0.066 0.097 0.205  


Trial #:2   Activation: No Activation    Date: 03/12/1984    Harvest Time: 26.8 hour(s)   Trial Call: Questionable   
  Dose
µg/mL
Total
Cells Examined
Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide
0         
100 3 0.030 2.0 0 0.000 0.0 3 0.030 2.0 0 0.000 0.0
Test Chemical: Azodicarbonamide
402         
100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0
498         
100 12 0.120 9.0 5 0.050 4.0 7 0.070 5.0 0 0.000 0.0
702         
100 3 0.030 2.0 2 0.020 2.0 1 0.010 1.0 0 0.000 0.0
798         
0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C
 0.025     
100 11 0.110 9.0 4 0.040 4.0 7 0.070 6.0 0 0.000 0.0
Mitomycin-C
 0.0625    
27 7 0.259 19.0 2 0.074 7.0 5 0.185 19.0 0 0.000 0.0
Trend: 0.499 1.345 -0.059  
Probability: 0.309 0.089 0.524  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 03/12/1984    Harvest Time: 18.8 hour(s)   Trial Call: Weakly Positive*   
  Dose
µg/mL
Total
Cells Examined
Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide
0         
100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: Azodicarbonamide
900         
100 3 0.030 3.0 0 0.000 0.0 3 0.030 3.0 0 0.000 0.0
1002         
100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
1104         
200 66 0.330 24.0 26 0.130 11.5 40 0.200 16.0 0 0.000 0.0
Positive Control: Cyclophosphamide
2.5       
100 7 0.070 7.0 2 0.020 2.0 5 0.050 5.0 0 0.000 0.0
Cyclophosphamide
12.5       
25 7 0.280 24.0 3 0.120 12.0 3 0.120 12.0 1 0.040 1.0
Trend: 6.328 4.577 4.983  
Probability: 0.000 0.000 0.000