Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01653-0008-0000-5
Chemical Name:	Anilazine
CASRN:	101-05-3
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G52321
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	103	113	69	22	25
			109	91	61	19
			73	96	72	33
Test Chemical	Anilazine	0.5	92	98	39	14	16
			99	117	52	18	16
		1	93	82	60	21	22
			75	80	52	23	22
		2	104	89	66	21	22
			96	96	63	22	22
		3	68	48	55	27	27
			116	64	94	27	27
		4	62	35	106	57	49*
			59	48	72	41	49*
		6	92	11	337	122	99*
			86	24	193	75	99*
Positive Control	Ethyl Methane Sulfonate	500	76	51	779	342	406*
			57	40	780	453
			61	39	775	422

Nonactivation Trial: 2 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	64	99	45	23	22
			61	82	42	23
			56	103	32	19
			75	116	46	20
Test Chemical	Anilazine	0.625	68	105	24	12	19
			48#	62	37.5	26	19
		1.25	53	84	34	21	20
			60	86	34	19	20
		2.5	50	70	37	25	24
			54	62	38	24	24
		3.75	47	51	27	19	19
			46	54	27	19	19
		5	54	28	83	52	95*
			36	6	151	139	95*
		7.5	48	6	178	124

		10

Positive Control	Ethyl Methane Sulfonate	500	38	51	558	487	483*
			60	64	700	387
			35	32	608	574

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	72	94	57	26	30
			74	92	73	33
			71	96	75	35
			76	119	62	27
Test Chemical	Anilazine	2	67	79	71	35	39
			67	67	87	43	39
		4	99	93	92	31	37
			103	81	130	42	37
		6	73	63	114	52	49*
			94	47	127	45	49*
		8	79	56	144	61	66*
			78	29	166	71	66*
		10	95	35	244	86	116*
			70	8	304	145	116*
		12	77	29	178	77	110*
			75	13	318	142	110*
		16

Positive Control	3-Methylcholanthrene	5	49	24	463	313	311*
			43	36	389	302
			47	31	452	318

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	66	98	87	44	41
			76	95	70	31
			65	111	86	44
			60	96	79	44
Test Chemical	Anilazine	0.94	98	128	79	27	29
			99	150	92	31	29
		1.88	77	106	63	27	25
			99	133	68	23	25
		3.75	88	95	62	23	26
			83	100	69	28	26
		7.5	72	69	73	34	34
			97	93	98	34	34
		12.5	52	12	131	84
		15	73	8	275	126	129*
			71	9	278	131	129*
Positive Control	3-Methylcholanthrene	5	56	29	578	343	426*
			46	26	658	477
			42	18	572	458

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination