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Study Information
Study Attribute Value
CEBS Accession Number: 002-01503-0002-0000-3
Chemical Name: 4-Hydroxyaminoquinoline-1-oxide
CASRN: 4637-56-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: G49031
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 78 105 101 43 45
    68 95 94 46
Test Chemical 4-Hydroxyaminoquinoline-1-oxide 
 0.0063    
 81 95 150 62 61
    70 89 125 60
  
 0.0125    
 77 95 108 47 60
    86 99 191 74
  
 0.025     
 79 100 243 102 105*
    62 79 198 107
  
 0.05      
 85 86 359 140 151*
    73 80 351 161
  
 0.1       
 74 67 394 177 185*
    67 64 389 193
  
 0.2       
 43 26 434 336 327*
    43 26 406 318
Positive Control Ethyl Methane Sulfonate 
500         
 35 33 696 666 681*
    34 30 716 695

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 89 109 90 34 37
    90 91 110 41
Test Chemical 4-Hydroxyaminoquinoline-1-oxide 
1.25      
 89 118 92 34 31
    90 114 74 28
  
2.5       
 98 141 106 36 44
    94 121 147 52
  
5         
 84 106 140 56 47
    91 116 105 38
  
10         
 82 101 192 78 76*
    88 109 196 74
  
20         
 72 86 372 173 153*
    82 97 330 134
  
40         
 54 41 364 224 206*
    57 44 320 188
Positive Control Ethyl Methane Sulfonate 
500         
 702*
    52 40 1088 702

Non-Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 85 103 103 40 43
    89 97 119 45
Test Chemical 4-Hydroxyaminoquinoline-1-oxide 
 0.23      
 78 95 194 83 74*
    76 101 148 65
  
 0.39      
 68 98 131 65 69
    90 99 198 73
  
 0.65      
 82 86 265 108 137*
    79 99 393 167
  
1.1       
 55 67 481 293 269*
    65 64 478 245
  
1.8       
 58 40 419 239 252*
    52 42 413 266
  
3         
 41 13 428 348 423*
    28 7 413 498
  
5         
   
Positive Control 3-Methylcholanthrene 
6         
 66 49 399 201 203*
    80 56 494 205

Non-Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 63 107 186 98 110
    63 93 229 121
Test Chemical 4-Hydroxyaminoquinoline-1-oxide 
 0.23      
 64 99 251 131 136
    60 104 253 142
  
 0.39      
 68 100 325 160 187*
    60 95 384 213
  
 0.65      
 52 69 397 252 270*
    53 76 455 288
  
1.1       
 39 30 344 297 358*
    33 27 416 420
  
1.8       
 2r 0 25 455
  
3         
   
Positive Control 3-Methylcholanthrene 
6         
 50 74 293 195 185*
    59 77 310 176

Non-Induced S9 Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 80 114 176 73 69
    84 111 194 77
    88 99 166 63
    90 76 173 64
Test Chemical 4-Hydroxyaminoquinoline-1-oxide 
 0.5       
 83 92 416 167 156*
    88 100 384 145
  
 0.7       
 45 26 543 401 311*
    69 49 453 220
  
 0.8       
 46 19 458 331 327*
    50 24 486 323
  
1         
 27 9 490 598 617*
    15 3 280 636
  
1.3       
 3r 0 24 267
    3r 0 50 556
Positive Control 3-Methylcholanthrene 
7         
 60 57 540 300 274*
    74 51 562 254
    73 53 586 269

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 111 109 64 19 18
    89 91 47 18
Test Chemical 4-Hydroxyaminoquinoline-1-oxide 
 0.38      
 79 95 126 54
  
 0.58      
 77 61 121 52 64*
    61 59 139 76
  
 0.89      
 61 46 129 70 79*
    58 36 153 88
  
1.37      
 47 19 196 138 141*
    49 19 214 145
  
2.11      
 22 5 190 290 258*
    25 7 170 227
Positive Control 3-Methylcholanthrene 
6         
 59 34 305 172 186*
    61 28 365 199

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 90 97 337 124 113
    93 103 284 102
Test Chemical 4-Hydroxyaminoquinoline-1-oxide 
 0.38      
 60 58 412 230 237*
    63 58 462 244
  
 0.58      
 59 49 499 280 295*
    58 50 538 309
  
 0.89      
 36 27 454 426 393*
    51 35 548 359
  
1.37      
 42 23 499 401 454*
    35 16 537 507
  
2.11      
 19 6 437 787 773*
    22 6 501 759
  
3.25      
 9r 2 287 1063
    8r 2 382 1661
Positive Control 3-Methylcholanthrene 
5         
 43 23 475 373 359*
    51 23 531 346

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination