Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-01503-0001-0000-2
Chemical Name:	4-Hydroxyaminoquinoline-1-oxide
CASRN:	4637-56-3
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G16836
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	110	134	47	14	14
Vehicle Control			116	71	51	15	14
Test Chemical	4-Hydroxyaminoquinoline-1-oxide	0.0156	89	32	101	38	45*
			77	22	119	52	45*
		0.0313	62	31	121	65	63*
			81	17	147	61	63*
		0.0625	80	16	265	111	93*
			84	31	189	75	93*
		0.125	63	10	273	145	146*
			60	18	263	146	146*
		0.25	35	7	241	227	256*
			45	7	382	285	256*
		0.5
			22	2	194	294
		1

Positive Control	Ethyl Methane Sulfonate	500	38	10	641	562	466*
Positive Control			59	27	656	371	466*
Negative Control	Negative (Not Specified)	0	97	129	50	17	14*
Negative Control			111	67	35	10	14*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	72	97	30	14	14
Vehicle Control			89	81	35	13	14
Test Chemical	4-Hydroxyaminoquinoline-1-oxide	0.0625	80	53	239	100	132*
			41	40	203	164	132*
		0.125	62	59	218	117	130*
			61	61	259	142	130*
		0.25	57	38	351	204	220*
			35#	25	244.5	235	220*
		0.5	24	4	208	289
			9r	1	125	472
		0.75	11r	1	113	359
			9r	1	80	291
		1

Positive Control	Ethyl Methane Sulfonate	500	80	79	608	255	263*
Positive Control			73	77	595	270	263*
Negative Control	Negative (Not Specified)	0	89	107	49	18	21*
Negative Control			86	115	59	23	21*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	90	91	59	22	22
			93	117	59	21
			74	68	46	21
			107	124	76	24
Test Chemical	4-Hydroxyaminoquinoline-1-oxide	0.01	84#	92	142.5	57	50*
			92	121	118	43	50*
		0.025	84	78	240	95	90*
			80	88	201	84	90*
		0.05	59	63	234	132	121*
			76	82	247	109	121*
		0.1	51	41	390	253	258*
			51	34	402	264	258*
		0.2	40	12	434	365	445*
			17	3	263	526	445*
		0.3	35	11	405	382	329*
			44	11	367	276	329*
		0.4	3r	0	59	621

		0.5

Positive Control	Ethyl Methane Sulfonate	500	32	16	772	817	735*
			48	37	791	549
			29	19	739	840

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	94	110	51	18	22
Vehicle Control			91	92	71	26	22
Test Chemical	4-Hydroxyaminoquinoline-1-oxide	0.0313	61	39	143	79	86*
			53	39	147	92	86*
		0.0625	63	57	232	123	120*
			61	50	215	118	120*
		0.125	52	26	333	212	210*
			51	32	316	208	210*
		0.25	27#	8	390	476	384*
			40	10	349	293	384*
		0.5	10r	1	134	432
			15r	1	142	309
		1

Positive Control	3-Methylcholanthrene	5	57	44	260	153	170*
Positive Control			64	32	360	188	170*
Negative Control	Negative (Not Specified)	0	86	83	64	25	22*
Negative Control			105	114	62	20	22*

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	87	93	36	14	19
			75	111	63	28
			67	84	34	17
			75	112	38	17
Test Chemical	4-Hydroxyaminoquinoline-1-oxide	0.0156	83	125	155	63	54*
			117	123	161	46	54*
		0.0313	62	85	192	104	102*
			67	73	199	100	102*
		0.0625	58	61	256	147	156*
			64	60	317	164	156*
		0.125	50	38	374	248	222*
			59	37	345	195	222*
		0.25	29	11	339	392	429*
			34	10	475	466	429*
		0.5	5r	0	105	778

		1

Positive Control	3-Methylcholanthrene	5	22	11	227	341	344*
			41	17	499	406
			39	13	331	284

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination