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Study Information
Study Attribute Value
CEBS Accession Number: 002-01494-0002-0000-2
Chemical Name: 4-Chloro-o-phenylenediamine
CASRN: 95-83-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 689355_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  Weakly Positive* 
Induced Rat Liver S9  1_S9  Negative 
No Activation  Positive 

Trial #:1   Activation: No Activation    Date: 01/23/1986    Harvest Time: 10.5 hour(s)   Trial Call: Weakly Positive*   
  Dose
µg/mL
Total
Cells Examined
Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide
0         
200 3 0.015 1.5 0 0.000 0.0 3 0.015 2.0 0 0.000 0.0
Test Chemical: 4-Chloro-o-phenylenediamine
1.61      
200 9 0.045 4.5 1 0.005 1.0 8 0.040 4.0 0 0.000 0.0
5.35      
200 12 0.060 5.0 0 0.000 0.0 12 0.060 5.0 0 0.000 0.0
16.1       
200 40 0.200 18.5 3 0.015 2.0 37 0.185 18.0 0 0.000 0.0
32.2       
0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C
1         
200 66 0.330 27.0 20 0.100 8.0 46 0.230 20.0 0 0.000 0.0
Mitomycin-C
5         
50 50 1.000 52.0 17 0.340 28.0 22 0.440 32.0 11 0.220 2.0
Trend: 6.236 1.799 5.991  
Probability: 0.000 0.036 0.000  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 01/23/1986    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL
Total
Cells Examined
Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide
0         
200 3 0.015 1.5 1 0.005 1.0 1 0.005 1.0 1 0.005 1.0
Test Chemical: 4-Chloro-o-phenylenediamine
32.2       
200 4 0.020 2.0 2 0.010 1.0 2 0.010 1.0 0 0.000 0.0
108         
200 1 0.005 0.5 1 0.005 1.0 0 0.000 0.0 0 0.000 0.0
322         
200 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
535         
0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide
50         
50 20 0.400 26.0 11 0.220 14.0 9 0.180 16.0 0 0.000 0.0
Trend: -1.961 -1.111 -1.823  
Probability: 0.975 0.867 0.966  


Trial #:2   Activation: No Activation    Date: 02/24/1986    Harvest Time: 20.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL
Total
Cells Examined
Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide
0         
200 5 0.025 2.5 0 0.000 0.0 5 0.025 3.0 0 0.000 0.0
Test Chemical: 4-Chloro-o-phenylenediamine
10.1       
50 71 1.420 32.0 3 0.060 6.0 8 0.160 16.0 60 1.200 6.0
12.7       
50 55 1.100 42.0 4 0.080 6.0 14 0.280 18.0 37 0.740 10.0
20.2       
50 87 1.740 48.0 5 0.100 8.0 19 0.380 28.0 63 1.260 9.0
Positive Control: Mitomycin-C
 0.1       
25 92 3.680 68.0 11 0.440 28.0 20 0.800 32.0 61 2.440 7.0
Mitomycin-C
5         
10 100 10.000 100.0 0 0.000 0.0 0 0.000 0.0 100 10.000 10.0
Trend: 8.786 3.531 5.633  
Probability: 0.000 0.000 0.000