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Study Information
Study Attribute Value
CEBS Accession Number: 002-01494-0001-0000-1
Chemical Name: 4-Chloro-o-phenylenediamine
CASRN: 95-83-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 689355_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  Positive 
No Activation  Positive 
Induced Rat Liver S9  1_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 01/21/1986    Trial Call: Positive   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide
0         
50 1044 458 0.439 9.16 26.5 0.000
Test Chemical 4-Chloro-o-phenylenediamine
 0.51      
50 1051 472 0.449 9.44 26.5 2.370
1.53      
50 1049 955 0.910 19.10 26.5 107.521
5.1       
50 1047 976 0.932 19.52 26.5 112.490
15.3       
0 0 0 0.000 0.00 30.5 N/A
Positive Control Mitomycin-C
 0.002     
50 1046 700 0.669 14.00 26.5 52.546
Mitomycin-C
 0.01      
10 209 288 1.378 28.80 26.5 214.110
Trend: 16.910        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 02/11/1986    Trial Call: Positive   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide
0         
50 1049 385 0.367 7.70 26.5 0.000
Test Chemical 4-Chloro-o-phenylenediamine
 0.76      
50 1035 500 0.483 10.00 26.5 31.627
5.05      
50 1032 927 0.898 18.54 26.5 144.746
7.6       
50 1027 1264 1.231 25.28 30.5 235.345
10.1       
0 0 0 0.000 0.00 30.5 N/A
Positive Control Mitomycin-C
 0.002     
50 1040 559 0.538 11.18 26.5 46.451
Mitomycin-C
 0.01      
10 206 206 1.000 20.60 26.5 172.468
Trend: 24.754        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 01/21/1986    Trial Call: Positive   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide
0         
50 1050 475 0.452 9.50 25.5 0.000
Test Chemical 4-Chloro-o-phenylenediamine
51         
50 1047 584 0.558 11.68 25.5 23.300
153         
50 1043 629 0.603 12.58 25.5 33.310
510         
50 1047 794 0.758 15.88 25.5 67.637
1530         
0 0 0 0.000 0.00 29.5 N/A
Positive Control Cyclophosphamide
 0.5       
50 1051 838 0.797 16.76 25.5 76.253
Cyclophosphamide
2.5       
10 210 444 2.114 44.40 25.5 367.368
Trend: 9.090        
Probability: 0.000