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Study Information
Study Attribute Value
CEBS Accession Number: 002-01494-0001-0000-1
Chemical Name: 4-Chloro-o-phenylenediamine
CASRN: 95-83-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 689355_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
No Activation  2  Positive 
Induced Rat Liver S9  1_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 01/21/1986    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1044 458 0.439 9.16 26.5 0.000
Test Chemical 4-Chloro-o-phenylenediamine 
 0.51      
 50 1051 472 0.449 9.44 26.5 2.370 
1.53      
 50 1049 955 0.910 19.10 26.5 107.521 
5.1       
 50 1047 976 0.932 19.52 26.5 112.490 
15.3       
 0 0 0 0.000 0.00 30.5 N/A
Positive Control Mitomycin-C 
 0.002     
 50 1046 700 0.669 14.00 26.5 52.546
Mitomycin-C 
 0.01      
 10 209 288 1.378 28.80 26.5 214.110
Trend: 16.910        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 02/11/1986    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1049 385 0.367 7.70 26.5 0.000
Test Chemical 4-Chloro-o-phenylenediamine 
 0.76      
 50 1035 500 0.483 10.00 26.5 31.627 
5.05      
 50 1032 927 0.898 18.54 26.5 144.746 
7.6       
 50 1027 1264 1.231 25.28 30.5 235.345 
10.1       
 0 0 0 0.000 0.00 30.5 N/A
Positive Control Mitomycin-C 
 0.002     
 50 1040 559 0.538 11.18 26.5 46.451
Mitomycin-C 
 0.01      
 10 206 206 1.000 20.60 26.5 172.468
Trend: 24.754        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 01/21/1986    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1050 475 0.452 9.50 25.5 0.000
Test Chemical 4-Chloro-o-phenylenediamine 
51         
 50 1047 584 0.558 11.68 25.5 23.300 
153         
 50 1043 629 0.603 12.58 25.5 33.310 
510         
 50 1047 794 0.758 15.88 25.5 67.637 
1530         
 0 0 0 0.000 0.00 29.5 N/A
Positive Control Cyclophosphamide 
 0.5       
 50 1051 838 0.797 16.76 25.5 76.253
Cyclophosphamide 
2.5       
 10 210 444 2.114 44.40 25.5 367.368
Trend: 9.090        
Probability: 0.000