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Study Information
Study Attribute Value
CEBS Accession Number: 002-01324-0001-0000-3
Chemical Name: 2-Ethylhexanoic acid
CASRN: 149-57-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 631517_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  Test Failure 
No Activation  Positive 
No Activation  Positive 
Induced Rat Liver S9  1_S9  Weakly Positive 
Induced Rat Liver S9  2_S9  Positive 
Induced Rat Liver S9  3_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 01/17/1984    Trial Call: Test Failure   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide
0         
50 1033 421 0.408 8.42 26.0 0.000
Test Chemical 2-Ethylhexanoic acid
313         
50 1036 457 0.441 9.14 26.0 8.237
Positive Control Mitomycin-C
 0.0015    
50 1022 672 0.658 13.44 26.0 61.338
Mitomycin-C
 0.01      
5 105 238 2.267 47.60 26.0 456.168
Trend: 0.000        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 01/31/1984    Trial Call: Positive   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide
0         
50 1040 447 0.430 8.94 26.0 0.000
Test Chemical 2-Ethylhexanoic acid
199         
50 1040 794 0.763 15.88 26.0 77.629
301         
50 1038 879 0.847 17.58 32.0 97.023
505         
50 1043 1217 1.167 24.34 32.0 171.476
2500         
0 0 0 0.000 0.00 32.0 N/A
Positive Control Mitomycin-C
 0.0015    
50 1036 670 0.647 13.40 26.0 50.467
Mitomycin-C
 0.01      
5 105 190 1.810 38.00 26.0 321.008
Trend: 18.655        
Probability: 0.000        

Trial #: 3    Activation: No Activation    Date: 03/05/1984    Trial Call: Positive   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide
0         
50 1038 470 0.453 9.40 26.0 0.000
Test Chemical 2-Ethylhexanoic acid
400         
50 1038 648 0.624 12.96 34.0 37.872
500         
50 1034 674 0.652 13.48 34.0 43.959
600         
50 1024 728 0.711 14.56 34.0 57.011
700         
50 1030 725 0.704 14.50 34.0 55.453
800         
50 1024 778 0.760 15.56 34.0 67.795
1000         
0 0 0 0.000 0.00 34.0 N/A
Positive Control Mitomycin-C
 0.0015    
50 1045 606 0.580 12.12 26.0 28.072
Mitomycin-C
 0.01      
5 99 203 2.051 40.60 26.0 352.856
Trend: 8.865        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 01/17/1984    Trial Call: Weakly Positive   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide
0         
50 1024 471 0.460 9.42 26.0 0.000
Test Chemical 2-Ethylhexanoic acid
9.38      
50 1037 427 0.412 8.54 26.0 -10.478
31.3       
50 1044 465 0.445 9.30 26.0 -3.165
93.8       
50 1034 634 0.613 12.68 26.0 33.305
313         
0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide
 0.4       
50 1034 668 0.646 13.36 26.0 40.454
Cyclophosphamide
2         
5 105 183 1.743 36.60 26.0 278.914
Trend: 5.030        
Probability: 0.000        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 01/31/1984    Trial Call: Positive   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide
0         
50 1042 587 0.563 11.74 26.0 0.000
Test Chemical 2-Ethylhexanoic acid
505         
50 1045 620 0.593 12.40 26.0 5.319
753         
50 1040 586 0.563 11.72 26.0 0.022
1000         
50 1038 733 0.706 14.66 26.0 25.353
2500         
50 1038 750 0.723 15.00 26.0 28.261
5200         
0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide
 0.4       
50 1043 699 0.670 13.98 26.0 18.966
Cyclophosphamide
2         
5 105 166 1.581 33.20 26.0 180.639
Trend: 5.319        
Probability: 0.000        

Trial #: 3_S9    Activation: Induced Rat Liver S9    Date: 02/29/1984    Trial Call: Positive   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide
0         
50 1031 414 0.402 8.28 26.0 0.000
Test Chemical 2-Ethylhexanoic acid
1000         
50 1036 501 0.484 10.02 26.0 20.430
1500         
50 1030 662 0.643 13.24 26.0 60.059
2100         
50 1023 605 0.591 12.10 33.0 47.278
3000         
50 1015 692 0.682 13.84 33.0 69.785
3500         
0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide
 0.4       
50 1032 568 0.550 11.36 26.0 37.065
Cyclophosphamide
2         
5 103 161 1.563 32.20 26.0 289.266
Trend: 9.104        
Probability: 0.000