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Study Information
Study Attribute Value
CEBS Accession Number: 002-01308-0002-0000-6
Chemical Name: 2-Chloro-p-phenylenediamine sulfate
CASRN: 61702-44-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 110183_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  Positive 
Induced Rat Liver S9  1_S9  Negative 
No Activation  Negative 
Induced Rat Liver S9  2_S9  Positive 
No Activation  Positive 

Trial #:1   Activation: No Activation    Date: 06/05/1985    Harvest Time: 17.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL
Total
Cells Examined
Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide
0         
100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: 2-Chloro-p-phenylenediamine sulfate
50         
100 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0 0 0.000 0.0
100         
100 11 0.110 10.0 6 0.060 6.0 5 0.050 5.0 0 0.000 0.0
150         
100 41 0.410 20.0 21 0.210 12.0 20 0.200 15.0 0 0.000 0.0
200         
100 125 1.250 45.0 66 0.660 32.0 45 0.450 28.0 14 0.140 5.0
Positive Control: Mitomycin-C
 0.0625    
100 60 0.600 42.0 40 0.400 31.0 20 0.200 17.0 0 0.000 0.0
Mitomycin-C
 0.25      
50 81 1.620 80.0 55 1.100 64.0 26 0.520 34.0 0 0.000 0.0
Trend: 8.944 7.154 7.242  
Probability: 0.000 0.000 0.000  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 04/10/1985    Harvest Time: 14.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL
Total
Cells Examined
Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide
0         
100 4 0.040 2.0 0 0.000 0.0 4 0.040 2.0 0 0.000 0.0
Test Chemical: 2-Chloro-p-phenylenediamine sulfate
 0.16      
100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
 0.3       
100 2 0.020 1.0 2 0.020 1.0 0 0.000 0.0 0 0.000 0.0
 0.5       
100 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide
7.5       
100 38 0.380 31.0 24 0.240 21.0 14 0.140 13.0 0 0.000 0.0
Cyclophosphamide
15         
50 34 0.680 52.0 21 0.420 32.0 13 0.260 22.0 0 0.000 0.0
Trend: -0.195 1.808 -1.824  
Probability: 0.577 0.035 0.966  


Trial #:2   Activation: No Activation    Date: 04/10/1985    Harvest Time: 13.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL
Total
Cells Examined
Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide
0         
100 2 0.020 1.0 0 0.000 0.0 2 0.020 1.0 0 0.000 0.0
Test Chemical: 2-Chloro-p-phenylenediamine sulfate
1.6       
100 4 0.040 4.0 1 0.010 1.0 3 0.030 3.0 0 0.000 0.0
3         
100 3 0.030 3.0 2 0.020 2.0 1 0.010 1.0 0 0.000 0.0
5         
100 3 0.030 3.0 2 0.020 2.0 1 0.010 1.0 0 0.000 0.0
Positive Control: Mitomycin-C
 0.125     
100 25 0.250 19.0 18 0.180 15.0 7 0.070 6.0 0 0.000 0.0
Mitomycin-C
 0.25      
50 19 0.380 32.0 12 0.240 20.0 7 0.140 14.0 0 0.000 0.0
Trend: 0.683 1.424 -0.383  
Probability: 0.247 0.077 0.649  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 04/10/1985    Harvest Time: 24.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL
Total
Cells Examined
Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide
0         
100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: 2-Chloro-p-phenylenediamine sulfate
1.6       
100 5 0.050 5.0 2 0.020 2.0 3 0.030 3.0 0 0.000 0.0
3         
100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
5         
100 12 0.120 9.0 5 0.050 5.0 7 0.070 6.0 0 0.000 0.0
10         
100 69 0.690 39.0 51 0.510 31.0 17 0.170 17.0 1 0.010 1.0
16         
100 64 0.640 31.0 34 0.340 21.0 30 0.300 22.0 0 0.000 0.0
30         
0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide
7.5       
100 52 0.520 34.0 38 0.380 27.0 14 0.140 13.0 0 0.000 0.0
Trend: 8.760 7.780 6.563  
Probability: 0.000 0.000 0.000  


Trial #:3   Activation: No Activation    Date: 04/10/1985    Harvest Time: 18.5 hour(s)   Trial Call: Positive   
  Dose
µg/mL
Total
Cells Examined
Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
No. of
Abs.
Abs
Per
Cell
% Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide
0         
100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0
Test Chemical: 2-Chloro-p-phenylenediamine sulfate
30         
100 11 0.110 9.0 3 0.030 3.0 8 0.080 6.0 0 0.000 0.0
50         
100 4 0.040 4.0 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0
100         
100 11 0.110 11.0 9 0.090 9.0 2 0.020 2.0 0 0.000 0.0
150         
100 45 0.450 18.0 26 0.260 12.0 9 0.090 8.0 10 0.100 1.0
200         
100 75 0.750 30.0 45 0.450 22.0 20 0.200 13.0 10 0.100 1.0
Positive Control: Mitomycin-C
 0.125     
100 46 0.460 33.0 21 0.210 19.0 15 0.150 14.0 10 0.100 1.0
Mitomycin-C
 0.25      
50 38 0.760 52.0 25 0.500 42.0 13 0.260 22.0 0 0.000 0.0
Trend: 6.064 6.160 2.919  
Probability: 0.000 0.000 0.002