Study Attribute | Value |
---|---|
CEBS Accession Number: | 002-01308-0002-0000-6 |
Chemical Name: | 2-Chloro-p-phenylenediamine sulfate |
CASRN: | 61702-44-1 |
NTP Study Type: | Genetic Toxicology - Mammalian Cell Cytogenetics |
NTP Study ID: | 110183_CA |
Study Result: | Positive |
Activation | Trial | Trial Call |
---|---|---|
No Activation | 1 | Positive |
Induced Rat Liver S9 | 1_S9 | Negative |
No Activation | 2 | Negative |
Induced Rat Liver S9 | 2_S9 | Positive |
No Activation | 3 | Positive |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Dimethyl Sulfoxide | 0 | 100 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 |
Test Chemical: | 2-Chloro-p-phenylenediamine sulfate | 50 | 100 | 2 | 0.020 | 2.0 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 |
100 | 100 | 11 | 0.110 | 10.0 | 6 | 0.060 | 6.0 | 5 | 0.050 | 5.0 | 0 | 0.000 | 0.0 | ||
150 | 100 | 41 | 0.410 | 20.0 | 21 | 0.210 | 12.0 | 20 | 0.200 | 15.0 | 0 | 0.000 | 0.0 | ||
200 | 100 | 125 | 1.250 | 45.0 | 66 | 0.660 | 32.0 | 45 | 0.450 | 28.0 | 14 | 0.140 | 5.0 | ||
Positive Control: | Mitomycin-C | 0.0625 | 100 | 60 | 0.600 | 42.0 | 40 | 0.400 | 31.0 | 20 | 0.200 | 17.0 | 0 | 0.000 | 0.0 |
Mitomycin-C | 0.25 | 50 | 81 | 1.620 | 80.0 | 55 | 1.100 | 64.0 | 26 | 0.520 | 34.0 | 0 | 0.000 | 0.0 | |
Trend: | 8.944 | 7.154 | 7.242 | ||||||||||||
Probability: | 0.000 | 0.000 | 0.000 |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Dimethyl Sulfoxide | 0 | 100 | 4 | 0.040 | 2.0 | 0 | 0.000 | 0.0 | 4 | 0.040 | 2.0 | 0 | 0.000 | 0.0 |
Test Chemical: | 2-Chloro-p-phenylenediamine sulfate | 0.16 | 100 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 |
0.3 | 100 | 2 | 0.020 | 1.0 | 2 | 0.020 | 1.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
0.5 | 100 | 2 | 0.020 | 2.0 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
Positive Control: | Cyclophosphamide | 7.5 | 100 | 38 | 0.380 | 31.0 | 24 | 0.240 | 21.0 | 14 | 0.140 | 13.0 | 0 | 0.000 | 0.0 |
Cyclophosphamide | 15 | 50 | 34 | 0.680 | 52.0 | 21 | 0.420 | 32.0 | 13 | 0.260 | 22.0 | 0 | 0.000 | 0.0 | |
Trend: | -0.195 | 1.808 | -1.824 | ||||||||||||
Probability: | 0.577 | 0.035 | 0.966 |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Dimethyl Sulfoxide | 0 | 100 | 2 | 0.020 | 1.0 | 0 | 0.000 | 0.0 | 2 | 0.020 | 1.0 | 0 | 0.000 | 0.0 |
Test Chemical: | 2-Chloro-p-phenylenediamine sulfate | 1.6 | 100 | 4 | 0.040 | 4.0 | 1 | 0.010 | 1.0 | 3 | 0.030 | 3.0 | 0 | 0.000 | 0.0 |
3 | 100 | 3 | 0.030 | 3.0 | 2 | 0.020 | 2.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | ||
5 | 100 | 3 | 0.030 | 3.0 | 2 | 0.020 | 2.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | ||
Positive Control: | Mitomycin-C | 0.125 | 100 | 25 | 0.250 | 19.0 | 18 | 0.180 | 15.0 | 7 | 0.070 | 6.0 | 0 | 0.000 | 0.0 |
Mitomycin-C | 0.25 | 50 | 19 | 0.380 | 32.0 | 12 | 0.240 | 20.0 | 7 | 0.140 | 14.0 | 0 | 0.000 | 0.0 | |
Trend: | 0.683 | 1.424 | -0.383 | ||||||||||||
Probability: | 0.247 | 0.077 | 0.649 |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Dimethyl Sulfoxide | 0 | 100 | 2 | 0.020 | 2.0 | 1 | 0.010 | 1.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 |
Test Chemical: | 2-Chloro-p-phenylenediamine sulfate | 1.6 | 100 | 5 | 0.050 | 5.0 | 2 | 0.020 | 2.0 | 3 | 0.030 | 3.0 | 0 | 0.000 | 0.0 |
3 | 100 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | 1 | 0.010 | 1.0 | 0 | 0.000 | 0.0 | ||
5 | 100 | 12 | 0.120 | 9.0 | 5 | 0.050 | 5.0 | 7 | 0.070 | 6.0 | 0 | 0.000 | 0.0 | ||
10 | 100 | 69 | 0.690 | 39.0 | 51 | 0.510 | 31.0 | 17 | 0.170 | 17.0 | 1 | 0.010 | 1.0 | ||
16 | 100 | 64 | 0.640 | 31.0 | 34 | 0.340 | 21.0 | 30 | 0.300 | 22.0 | 0 | 0.000 | 0.0 | ||
30 | 0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | 0 | 0.000 | 0.0 | ||
Positive Control: | Cyclophosphamide | 7.5 | 100 | 52 | 0.520 | 34.0 | 38 | 0.380 | 27.0 | 14 | 0.140 | 13.0 | 0 | 0.000 | 0.0 |
Trend: | 8.760 | 7.780 | 6.563 | ||||||||||||
Probability: | 0.000 | 0.000 | 0.000 |
Dose µg/mL | Total Cells Examined | Total Aberrations | Complex Aberrations | Simple Aberrations | Other Abs | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of Abs | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | No. of Abs. | Abs Per Cell | % Cells With Abs | ||||
Vehicle Control: | Dimethyl Sulfoxide | 0 | 100 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 |
Test Chemical: | 2-Chloro-p-phenylenediamine sulfate | 30 | 100 | 11 | 0.110 | 9.0 | 3 | 0.030 | 3.0 | 8 | 0.080 | 6.0 | 0 | 0.000 | 0.0 |
50 | 100 | 4 | 0.040 | 4.0 | 2 | 0.020 | 2.0 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 | ||
100 | 100 | 11 | 0.110 | 11.0 | 9 | 0.090 | 9.0 | 2 | 0.020 | 2.0 | 0 | 0.000 | 0.0 | ||
150 | 100 | 45 | 0.450 | 18.0 | 26 | 0.260 | 12.0 | 9 | 0.090 | 8.0 | 10 | 0.100 | 1.0 | ||
200 | 100 | 75 | 0.750 | 30.0 | 45 | 0.450 | 22.0 | 20 | 0.200 | 13.0 | 10 | 0.100 | 1.0 | ||
Positive Control: | Mitomycin-C | 0.125 | 100 | 46 | 0.460 | 33.0 | 21 | 0.210 | 19.0 | 15 | 0.150 | 14.0 | 10 | 0.100 | 1.0 |
Mitomycin-C | 0.25 | 50 | 38 | 0.760 | 52.0 | 25 | 0.500 | 42.0 | 13 | 0.260 | 22.0 | 0 | 0.000 | 0.0 | |
Trend: | 6.064 | 6.160 | 2.919 | ||||||||||||
Probability: | 0.000 | 0.000 | 0.002 |