Study Attribute | Value |
---|---|
CEBS Accession Number: | 002-01308-0001-0000-5 |
Chemical Name: | 2-Chloro-p-phenylenediamine sulfate |
CASRN: | 61702-44-1 |
NTP Study Type: | Genetic Toxicology - Mammalian Cell Cytogenetics |
NTP Study ID: | 110183_SCE |
Study Result: | Positive |
Activation | Trial | Trial Call |
---|---|---|
No Activation | 1 | Positive |
No Activation | 2 | Positive |
Induced Rat Liver S9 | 1_S9 | Weakly Positive |
Induced Rat Liver S9 | 2_S9 | Questionable |
Dose µg/mL | Number Cells Examined | Number Chromosomes Examined | Total Number SCEs | SCE / Chromosome | SCE / Cell | Hours in BRDU | % Increase Over Solvent Control | ||
---|---|---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 50 | 1045 | 477 | 0.456 | 9.54 | 26.0 | 0.000 |
Test Chemical | 2-Chloro-p-phenylenediamine sulfate | 0.5 | 50 | 1040 | 547 | 0.526 | 10.94 | 26.0 | 15.226 |
1.6 | 50 | 1044 | 580 | 0.556 | 11.60 | 26.0 | 21.710 | ||
5 | 50 | 1044 | 587 | 0.562 | 11.74 | 26.0 | 23.179 | ||
16 | 50 | 1044 | 776 | 0.743 | 15.52 | 26.0 | 62.839 | ||
50 | 0 | 0 | 0 | 0.000 | 0.00 | 26.0 | N/A | ||
Positive Control | Mitomycin-C | 0.001 | 50 | 1043 | 659 | 0.632 | 13.18 | 27.0 | 38.420 |
Mitomycin-C | 0.005 | 10 | 210 | 243 | 1.157 | 24.30 | 27.0 | 153.504 | |
Trend: | 8.271 | ||||||||
Probability: | 0.000 |
Dose µg/mL | Number Cells Examined | Number Chromosomes Examined | Total Number SCEs | SCE / Chromosome | SCE / Cell | Hours in BRDU | % Increase Over Solvent Control | ||
---|---|---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 50 | 1047 | 430 | 0.411 | 8.60 | 26.5 | 0.000 |
Test Chemical | 2-Chloro-p-phenylenediamine sulfate | 1.6 | 50 | 1049 | 516 | 0.492 | 10.32 | 26.5 | 19.771 |
5 | 50 | 1049 | 636 | 0.606 | 12.72 | 26.5 | 47.625 | ||
16 | 50 | 1049 | 694 | 0.662 | 13.88 | 26.5 | 61.088 | ||
50 | 0 | 0 | 0 | 0.000 | 0.00 | 26.5 | N/A | ||
50 | 50 | 1050 | 1100 | 1.048 | 22.00 | 31.5 | 155.083 | ||
Positive Control | Mitomycin-C | 0.001 | 50 | 1049 | 597 | 0.569 | 11.94 | 26.5 | 38.573 |
Mitomycin-C | 0.005 | 10 | 209 | 259 | 1.239 | 25.90 | 26.5 | 201.739 | |
Trend: | 18.581 | ||||||||
Probability: | 0.000 |
Dose µg/mL | Number Cells Examined | Number Chromosomes Examined | Total Number SCEs | SCE / Chromosome | SCE / Cell | Hours in BRDU | % Increase Over Solvent Control | ||
---|---|---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 50 | 1043 | 400 | 0.384 | 8.00 | 26.0 | 0.000 |
Test Chemical | 2-Chloro-p-phenylenediamine sulfate | 0.16 | 50 | 1046 | 409 | 0.391 | 8.18 | 26.0 | 1.957 |
0.5 | 50 | 1045 | 500 | 0.478 | 10.00 | 26.0 | 24.761 | ||
1.6 | 0 | 0 | 0 | 0.000 | 0.00 | 26.0 | N/A | ||
Positive Control | Cyclophosphamide | 0.3 | 50 | 1033 | 675 | 0.653 | 13.50 | 26.0 | 70.384 |
Cyclophosphamide | 0.6 | 10 | 207 | 212 | 1.024 | 21.20 | 26.0 | 167.048 | |
Trend: | 3.353 | ||||||||
Probability: | 0.000 |
Dose µg/mL | Number Cells Examined | Number Chromosomes Examined | Total Number SCEs | SCE / Chromosome | SCE / Cell | Hours in BRDU | % Increase Over Solvent Control | ||
---|---|---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 50 | 1042 | 454 | 0.436 | 9.08 | 26.0 | 0.000 |
Test Chemical | 2-Chloro-p-phenylenediamine sulfate | 0.016 | 50 | 1048 | 405 | 0.386 | 8.10 | 26.0 | -11.304 |
0.05 | 50 | 1049 | 409 | 0.390 | 8.18 | 26.0 | -10.513 | ||
0.16 | 50 | 1047 | 457 | 0.436 | 9.14 | 26.0 | 0.180 | ||
0.5 | 50 | 1047 | 511 | 0.488 | 10.22 | 26.0 | 12.018 | ||
1 | 50 | 1040 | 517 | 0.497 | 10.34 | 26.0 | 14.096 | ||
1.6 | 0 | 0 | 0 | 0.000 | 0.00 | 31.0 | N/A | ||
Positive Control | Cyclophosphamide | 0.3 | 50 | 1051 | 823 | 0.783 | 16.46 | 26.0 | 79.725 |
Cyclophosphamide | 0.6 | 10 | 210 | 213 | 1.014 | 21.30 | 26.0 | 132.794 | |
Trend: | 3.890 | ||||||||
Probability: | 0.000 |