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Study Information
Study Attribute Value
CEBS Accession Number: 002-01308-0001-0000-5
Chemical Name: 2-Chloro-p-phenylenediamine sulfate
CASRN: 61702-44-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 110183_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  Positive 
No Activation  Positive 
Induced Rat Liver S9  1_S9  Weakly Positive 
Induced Rat Liver S9  2_S9  Questionable 

Trial #: 1    Activation: No Activation    Date: 02/07/1985    Trial Call: Positive   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide
0         
50 1045 477 0.456 9.54 26.0 0.000
Test Chemical 2-Chloro-p-phenylenediamine sulfate
 0.5       
50 1040 547 0.526 10.94 26.0 15.226
1.6       
50 1044 580 0.556 11.60 26.0 21.710
5         
50 1044 587 0.562 11.74 26.0 23.179
16         
50 1044 776 0.743 15.52 26.0 62.839
50         
0 0 0 0.000 0.00 26.0 N/A
Positive Control Mitomycin-C
 0.001     
50 1043 659 0.632 13.18 27.0 38.420
Mitomycin-C
 0.005     
10 210 243 1.157 24.30 27.0 153.504
Trend: 8.271        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 04/30/1985    Trial Call: Positive   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide
0         
50 1047 430 0.411 8.60 26.5 0.000
Test Chemical 2-Chloro-p-phenylenediamine sulfate
1.6       
50 1049 516 0.492 10.32 26.5 19.771
5         
50 1049 636 0.606 12.72 26.5 47.625
16         
50 1049 694 0.662 13.88 26.5 61.088
50         
0 0 0 0.000 0.00 26.5 N/A
50         
50 1050 1100 1.048 22.00 31.5 155.083
Positive Control Mitomycin-C
 0.001     
50 1049 597 0.569 11.94 26.5 38.573
Mitomycin-C
 0.005     
10 209 259 1.239 25.90 26.5 201.739
Trend: 18.581        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 02/07/1985    Trial Call: Weakly Positive   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide
0         
50 1043 400 0.384 8.00 26.0 0.000
Test Chemical 2-Chloro-p-phenylenediamine sulfate
 0.16      
50 1046 409 0.391 8.18 26.0 1.957
 0.5       
50 1045 500 0.478 10.00 26.0 24.761
1.6       
0 0 0 0.000 0.00 26.0 N/A
Positive Control Cyclophosphamide
 0.3       
50 1033 675 0.653 13.50 26.0 70.384
Cyclophosphamide
 0.6       
10 207 212 1.024 21.20 26.0 167.048
Trend: 3.353        
Probability: 0.000        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 04/30/1985    Trial Call: Questionable   
  Dose
µg/mL
Number
Cells
Examined
Number
Chromosomes
Examined
Total
Number
SCEs
SCE /
Chromosome
SCE /
Cell
Hours in
BRDU
% Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide
0         
50 1042 454 0.436 9.08 26.0 0.000
Test Chemical 2-Chloro-p-phenylenediamine sulfate
 0.016     
50 1048 405 0.386 8.10 26.0 -11.304
 0.05      
50 1049 409 0.390 8.18 26.0 -10.513
 0.16      
50 1047 457 0.436 9.14 26.0 0.180
 0.5       
50 1047 511 0.488 10.22 26.0 12.018
1         
50 1040 517 0.497 10.34 26.0 14.096
1.6       
0 0 0 0.000 0.00 31.0 N/A
Positive Control Cyclophosphamide
 0.3       
50 1051 823 0.783 16.46 26.0 79.725
Cyclophosphamide
 0.6       
10 210 213 1.014 21.30 26.0 132.794
Trend: 3.890        
Probability: 0.000